Active Ingredients: Norfloxacin
Concentrations of pefloxacin and its major metabolite N-demethyl pefloxacin norfloxacin were measured by use of high-performance liquid chromatography.
Amrita Nayak and V. Molecular Pharmaceutics . Grothe. Meekes. Vlieg.
Varin Titapiwatanakun, Abdul W. Basit, and Simon Gaisford. Luis Padrela, Miguel A. Velaga, Henrique A. Matos, and Edmundo Gomes de Azevedo. Juan M. Scott C. This absorption increases the amplitude of vibrational motion of the chemical bonds.
In addition to the absence of organic solvents, the spectrophotometric method in the infrared region has other advantages, such as being a rapid technique that does not require a pretreatment of the sample and assisting in detecting impurities.
Another advantage is the low cost of materials for the manufacture of pellets. On the other hand, this method requires high control of humidity and temperature of the working environment.
The development of the analytical methods by spectrophotometry in the mid-infrared region has been successfully used in the quantification of other drugs. To develop the proposed method, the first step was a qualitative test.
For this, an overlap of the spectra obtained with amoxicillin reference standard and amoxicillin in capsules was carried out Figure 1. It was noticeable that both spectra have the same absorption bands, confirming the absence of adjuvants interference, impurities, or degradation products.
The similarity between the spectra is a strong indicative of the identity of amoxicillin in the pharmaceutical dosage form.
The second step for the development of the quantitative method was to select a spectral range in the spectrum of amoxicillin in the mid-infrared region, to be quantitatively analyzed. For this, a comparison between the spectrum obtained with amoxicillin reference standard and each adjuvant present in the composition of amoxicillin in capsules was performed Figure 2.
Thus, this was the spectral range selected for the quantitative analysis.